Allelic differences affecting invariant chain dependency of MHC class II subunit assembly

Class II-associated invariant chain peptide-independent binding of invariant chain to class II MHC molecules.

The class II-associated invariant chain peptide (CLIP) region of invariant chain (Ii) is believed to play a critical role in the assembly and transport of MHC class II alphabetaIi complexes through its interaction with the class II peptide-binding site. The role of the CLIP sequence was investigated by using mutant Ii molecules with altered affinity for the DR1 peptide-binding site. Both high- ...

Invariant Chain Structure and MHC Class II Function

Peter Cresswell The model as set out above raises some interesting Howard Hughes Medical Institute structural and mechanistic questions. First, what are the Section of Immunobiology structural properties of the Ii chain that allow it to interact Yale University School of Medicine with class II molecules in a way that prevents them New Haven, Connecticut 06520-8011 from binding peptides? Second,...

Shark class II invariant chain reveals ancient conserved relationships with cathepsins and MHC class II.

The invariant chain (Ii) is the critical third chain required for the MHC class II heterodimer to be properly guided through the cell, loaded with peptide, and expressed on the surface of antigen presenting cells. Here, we report the isolation of the nurse shark Ii gene, and the comparative analysis of Ii splice variants, expression, genomic organization, predicted structure, and function throu...

Sorting of MHC class II molecules and the associated invariant chain (Ii) in polarized MDCK cells.

Epithelial cells have been found to express MHC class II molecules in vivo and are able to perform class II-restricted antigen presentation. The precise intracellular localization of these molecules in epithelial cells has been a matter of debate. We have analyzed the polarized targeting of human MHC class II molecules and the associated invariant chain (Ii) in stably transfected MDCK cells. Th...

Class II Allotypes Promiscuous Binding of Invariant Chain to MHC Identification of a Sequence That Mediates